1. In-vivo measurement of arterial stiffness was conducted on 125 volunteers using ARTSENS Desktop (in a sitting posture) and Aloka Prosound α10 imaging system (in sitting and supine posture).

2. Blood samples were drawn from all volunteers to measure cholesterol and blood sugar levels, and anthropometric data were also recorded.

3. The carotid-radial pulse wave velocity was also measured on all the male subjects included in the study.

1. Data analysis of 111 subjects (after the outlier elimination) depicted a strong correlation between carotid stiffness indices measured by ARTSENS and those measured by the imaging system.

2. The correlation co-efficient (r) for, β was 0.9, while that for AC was 0.7, and for E_P was 0.94.

3. The mean difference between the β measurements given by the imaging system and ARTSENS was found to be 0.06 ± 1.04, indicating an insignificant bias.

4. Bland-Altman analysis revealed the difference to be randomly distributed, with 84% of the measurements within ± 1.5 SD. It was also found that 99% of all the measurements fell within mean ± 2 SD, within acceptable limits of variation.

5. A comparison of the stiffness measurements made by ARTSENS on the subject in sitting posture with those made by the imaging system with the subject in supine position also demonstrated strong correlation (r = 0.9 for β, r = 0.8 for AC, and r = 0.9 for E_P).

6. Deming’s regression analysis demonstrated that there was no bias in the measurement of β and E_P.

7. A strong trend of increased arterial stiffness with age was also observed, illustrating the ability of the device to detect age-related variations in arterial wall properties (age trends were analyzed after eliminating subjects on medication).